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Paper Title: Effect of Annona Muricata on CD8 and Granzyme-b Expression in Severe Plasmodium berghei Anka Infected Swiss Mice
Authors Name: Abdulkarim Abdullah Mohammed Amammish , Kis Djamiatun , Muflihatul Muniroh , Dik Puspasari
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Published Paper Id: IJNRD2008038
Published In: Volume 5 Issue 8, August-2020
Abstract: Several studies have reported the role of T cells in Plasmodium berghei ANKA (PbA) that related with the blood and liver patology. Granzyme B (GzmB) is a serine protease released by CD8+ T cells and natural killer cells during cellular immune response, The purpose of these study to determine the effect of Annona muricata leaf-ethanolic exract (AME) on GzmB and CD8-expression-score in spleens of PbA-infected-Swiss-mice. This experimental study used randomized post test only control group design. Thirty six female Swiss mice were divided into 6 groups. C1-group was control-healthy mice, G1 and G2-groups were healthy-mice treated with AME dosage 100 and 150 mg/kg BW/day for 14-day-period. C2-group was control-PbA-infected-group. G3 and G4-groups received AME dosage 100 and 150 mg/kg BW, respectively for 7-day-pre and post-PbA-infection. GzmB and CD8 in the spleen stained by immunohistochemistry-method, then two-pathology-anatomy-experts determined Allred-score by using light microscope. Statistical analyzes used were One-way-ANOVA-test followed by post-hoc-test, and. Pearson-correlation-test. G4 and C1-groups had no significant different GzmB-expression-score (5.60 ± 0.46 and 4.10 ± 1.27; p = 0.134), while those of G3 and C2-groups (6.03 ± 0.48 and 6.70 ± 0.99) were significantly higher than C1-group (p = 0.015 and p = 0.012). AME (150mg/kg BW/day)-treatment associated with GzmB-expression-normal score during severe-PbA-infection. G3, G4 and C2-groups (5.03 ± 0.79; 6.20 ± 1.46 and 6.60 ± 2.77) had no significant different CD8-expression-score compare to C1-group (3.80 ± 2.60; p = 0.829; p = 0.284 and p = 0.150). The correlation score of GzmB and CD8 expression was not found in healthy Swiss mice infected with PbA (r = -0.248; p = 0.158). AME in dose of 150 mg/kg BW/day-treatment normalize GzmB-expression-score of severe-PbA-infected-Swiss-mice, however AME at any dose studied have no influence on CD8- expression-score of those mice
Keywords: Annona muricata, P. berghei Anka, granzyme B, CD8
Cite Article: "Effect of Annona Muricata on CD8 and Granzyme-b Expression in Severe Plasmodium berghei Anka Infected Swiss Mice", International Journal of Novel Research and Development (, ISSN:2456-4184, Vol.5, Issue 8, page no.338-346, August-2020, Available :
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