INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016
Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI)
A simple, accurate, precise, specific Reverse phase High performance liquid chromatography method was developed and validated for simultaneous estimation of lidocaine, nifedipine, and imidazolidinyl urea in pharmaceutical formulation. The approach was validated for lidocaine, nifedipine, and imidazolidinyl urea using ICH recommendation over a range of 15-75 µl/mL and 3-15 µl/mL and 2-10 µl/mL for lidocaine, nifedipine, imidazolidinyl urea respectively. An analytical column HYPERSIL ODS C18250 nm * 4.6 mm was utilized. At a flow rate of 1.0 ml/min. the mobile phase was Acetonitrile:methanol:0.05m potassium dihydrogen phosphate buffer(40:35:25v/v) ratio. The elution was examined using UV detector with detection wavelength of 225 nm. The retention time for lidocaine, nifedipine, and imidazolidinyl urea are 4.091min, 8.291min and 5.521min respectively. Accuracy, precision, repeatability, robustness and specificity in accordance with ICH guidelines. The method can be successfully employed for the simultaneous determination of lidocaine, nifedipine and imidazolidinyl urea in pharmaceutical formulation
Keywords:
RP HPLC METHOD, LIDOCAINE, NIFEDIPINE, IMIDAZOLIDIYL UREA VALIDATION ICH Q2 R1
Cite Article:
"RP-HPLC METHOD DEVLOPMENT VALIDATION FOR SIMULTANEOUS ESTIMATION OF LIDOCAINE,NIFEDIPINE AND IMIDAZOLIDINYL UREA IN THE TOPICAL DOSAGE FORM", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.8, Issue 5, page no.h681-h700, May-2023, Available :http://www.ijnrd.org/papers/IJNRD2305790.pdf
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ISSN:
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
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