INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016
Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI)
Sirolimus (Rapamycin) is a coveted drug substance obtained from actinobacteria named Streptomyces hygroscopicus, which synthesizes it as a secondary metabolite. It has been studied in regard to a variety of biological conditions such as anti-pathogenicity, immunosuppression, anti-aging, and anti-cancerous activity. Devising methodologies to obtain a sufficient amount of sirolimus is a part of eminent research prospects. For the current study, enhancement in sirolimus production has been achieved by following a sequential regime involving improvement in the fermentation media and mutation of the parent strain along with careful and continuous screening at each step. The investigation encompasses the establishment of initial production activity followed by optimization of medium components using Plackett-Burman design and OVAT methodology. The significant components obtained have been thoroughly monitored to finalize the concentration which yielded maximum production of sirolimus. Then the procured strain of Streptomyces hygroscopicus has been mutated using intermittent treatment with UV radiations. The stable culture colonies obtained thereby have been taken for chemical mutagenesis using NTG. The colonies offering stable and enhanced yield have been isolated to inoculate the optimized media. Finally, 2.5 to 2.8 - fold augmentation in the production of sirolimus has been observed concerning the initially recorded activity.
"SIROLIMUS: PRODUCTION ENHANCEMENT USING MUTAGENESIS AND MEDIUM OPTIMISATION", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.7, Issue 1, page no.100-108, January-2022, Available :http://www.ijnrd.org/papers/IJNRD2201015.pdf
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2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
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