INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016
Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI)
Code:60/2022/1
Invitro Regeneration from Stem node explants of Celestrus paniculatrus(Wild)
Mandaloju Venkateshwarlu
MSc.,Ph.D.,Botany,MSc.,Biotechnology,FHAS,SFS,SFAB,CFE and LLB
Department of Botany, Kakatiya University, Warangal Urabn – 506 009, T.S. INDIA
Email: drvenkat6666@gmail.com.
ABSTRACT
The primary aim of this study have been to gain some knowledge about the genotypic differnces for callus induction and high frequency plant regeneration stem node segments of Celestrus paniculatrus were inoculated on MS basal medium supplemented with various cytokines BAP and NAA Coconut water also had a role in triggering the formation of multiple shoots. Addition of BAP at 2.0 mg/l and NAA at 3.0 mg/l to the MS basal medium, induced regeneration from Stem node expalnts with an increase in the level of BAP 2.0 – 4.0 mg/l the percentage of explants producing shoots also increased. The number of shoots developed on the Stem node expalnts ranged from 1-4 to 2-3 by the addition of BAP at concentration of 2.0 mg/l or NAA at 3.0 mg/l. Among the three concentrations of coconut milk used i.e., 10,15 and 20% , 15% of coconut milk along with 0.5 mg/l BAP + Kn proved to be ideal for multiple shoot induction. MS medium fortified with 1.0 mg/l BAP or 2.0 mg/l L-G Glutamic acid also induced shoot buds on Stem node explants. Plant tissue culture and callus induction from cotyledonary segments,M.Venkateshwarlu(2021).
"Invitro Regeneration from Stem node explants of Celestrus paniculatrus(Wild)", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.7, Issue 1, page no.125-128, January-2022, Available :http://www.ijnrd.org/papers/IJNRD2201019.pdf
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ISSN:
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
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