Forensic analysis of traces of bromadiolone rodenticide from soil at site where biological flesh sample buried

Pratiksha Pise , Anurag Sahu

Bromadiolone, Rodenticide, Rat poison, TLC, soil

Bromadiolone is second generation single dose anticoagulant rodenticide like zinc phosphide. It is widely used to eradicate rodent from affected places and easily available in the form of the rat kill cake of various manufacturer i.e. Mortein , Tiger Mortein, Ratol etc. in Indian market. As a effective rodenticide, it is increasingly being used by people for committing suicide and even homicide. Authors conducted this experiment for the study of traces of bromadiolone in soil samples after the complete decomposition of the soft tissue where buried for forensic purpose. In this paper authors selected a Mortein rat kill bait of 25gm(0.005% of bromadiolone) five in numbers. A total 125gm of powdered rat kill cake subjected to 0.125gm of goat flesh without bone at the room temperature for one hour. This soft tissue of the flesh placed on the plastic pot between the layers of soil (pH -7.5) at the room temperature. At every seven days of completion (i.e. 7,14,21,28 days) of the same sample has been observed for the presence of the maggot, pupa, colour, smell and stage of decomposition. And after the 28 days since 21 November 2019, soil samples taken out from pot and analyzed by Thin layer chromatography. Traces of bromadiolone from soil samples confirmed by TLC. Blue and brown spot has been developed and their Rf×1oo values measured as 90 & 45 respectively.

Published Paper   E-Certificate

"Forensic analysis of traces of bromadiolone rodenticide from soil at site where biological flesh sample buried", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.8, Issue 3, page no.a790-a798, March-2023, Available :https://ijnrd.org/papers/IJNRD2303082.pdf

Volume 8 Issue 3, March-2023

Pages : a790-a798

Paper Reg. ID: IJNRD_188303

Published Paper Id: IJNRD2303082

Downloads: 000118850

Research Area: Chemistry

Country: Chennai, Tamil Nadu, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2303082

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2303082

DOI: https://doi.org/10.5281/zenodo.13692366

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJNRD (IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition