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IJNRD
INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT
International Peer Reviewed & Refereed Journals, Open Access Journal
ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016
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Impact Factor : 8.76

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Paper Title: Isolation, Identification and Characterization of Bacteria of Coal Mine Soil at Pandwa of Rajhara Coalfield, Jharkhand
Authors Name: Manoj Kumar Singh , Dr. K.K Mishra
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IJNRD_196110
Published Paper Id: IJNRD2305572
Published In: Volume 8 Issue 5, May-2023
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Abstract: Mining is the process of extracting useful materials from the earth. The dust and noxious fumes inhaled by miners make them vulnerable to pulmonary diseases. The water sources in the region get contaminated due to mining. Dumping of waste and slurry leads to degradation of land, soil, and increase in stream and river pollution. Mining means any naturally occurring substance from earth for useful purposes. During this mining process, several changes take place in the physical, chemical, and microbiological characteristics of soil as a result of storage. Soil is a system, in which constant interface between soil minerals and microorganisms control the physicochemical and biological properties of global ecosystem. Microbial properties have been reported to be useful indicators of soil quality and could possibly serve as assessment criteria of successful rehabilitation of ecologically disturbed areas. The present study was conducted to determine dominant bacterial species in the soils of Sonepur Bazari at Raniganj coalfield. During this study, the soil samples were collected in different sites and the bacteria was isolated, identified biochemically and characterized by phylogenetic analysis using 16SrRNA sequencing technique. Two bacterial isolates, i.e. S-1 and S-2 were obtained by using Nutrient agar medium. The isolates were identified morphologically, biochemically and also by phylogenetic analysis using 16SrRNA sequencing technique. The molecular identification of 16SrRNA gene sequences showed that the isolates had 95% similarity to the genus. The results showed that between the two different strains Bacillus cereus is a common soil bacteria whereas Bhargavaea cecembensis is a newly found bacteria in soil.
Keywords: Bacillus cereus, Bhargavaea, cecembensis, Rajhara Coalfield, 16S rRNA, Analysis, soil bacteria
Cite Article: "Isolation, Identification and Characterization of Bacteria of Coal Mine Soil at Pandwa of Rajhara Coalfield, Jharkhand", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.8, Issue 5, page no.f470-f476, May-2023, Available :http://www.ijnrd.org/papers/IJNRD2305572.pdf
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ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publication Details: Published Paper ID:IJNRD2305572
Registration ID: 196110
Published In: Volume 8 Issue 5, May-2023
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Page No: f470-f476
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Research Area: Engineering
Publisher : IJ Publication
Published Paper URL : https://www.ijnrd.org/viewpaperforall?paper=IJNRD2305572
Published Paper PDF: https://www.ijnrd.org/papers/IJNRD2305572
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ISSN: 2456-4184
Impact Factor: 8.76 and ISSN APPROVED
Journal Starting Year (ESTD) : 2016

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