Paper Title
RESEARCH ON METHOD DEVELOPMENT AND VALIDATION OF DECLATASVIR DIHYDROCHLORIDE BY RP HPLC METHOD
Article Identifiers
: Authors
Keywords
Daclatasvir, Dihydrochloride , UV, HPLC, Method validation.
Abstract
Daclatasvir HCl is an antiviral drug. Daclatasvir dihydrochloride is the most frequently used as antiviral drug for a treatment of hepatitis C virus. Daclatasvir HCl prevents RNA replication and virion assembly by binding to NS5A, a nonstructural phosphor protein encoded by HCV Daclatasvir HCl efficient in vitro against viral strains less sensitive to other PIs. Present investigation involves the development and validation of chromatographic method for Daclatasvir HCl in bulk drug as well as pharmaceutical dosage form as per ICH guideline. The spectrophotometric determination was performed in UV Shimadzo, Model: UV-1800. The separation was conducted by Methanol. The wavelength was found at wavelength 315 nm. Chromatographic determination was performed on Agilent 1220 Infinity LC with ezchrome software with variable wavelength detector. The separation was conducted by using Zobrax Eclipse XDB-C 18 (4.6×150×5µm) with mobile phase consisting of acetonitrile: methanol: water in the ratio (60:30:10). The mobile phase was delivered at the flow rate of 1.0 ml/min. The eluent was monitored at wavelength 315 nm by variable wavelength detector and found a sharp and symmetrical peak with retention time 3.5.The method was validated for linearity, accuracy, precision, system suitability, and stability. The method was found to be linear over the concentration range 2-12µg/ml with coefficient regression R2 -0.999.The obtained results proved the method can be employed for the routine analysis of Daclatasvir HCl in bulk drug as well as in a commercial formulations.
Downloads
How To Cite (APA)
Miss. Trupti M.Haral, Miss. Monali moreshwar Sawarbandhe, Mr. Prajyot Balu Ravat, Miss. Dipika Jagdish Joshi, & Miss.Warad Tanuja Annarao (October-2024). RESEARCH ON METHOD DEVELOPMENT AND VALIDATION OF DECLATASVIR DIHYDROCHLORIDE BY RP HPLC METHOD. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(10), a770-a797. https://ijnrd.org/papers/IJNRD2410082.pdf
Issue
Volume 9 Issue 10, October-2024
Pages : a770-a797
Other Publication Details
Paper Reg. ID: IJNRD_301110
Published Paper Id: IJNRD2410082
Downloads: 000122030
Research Area: Pharmacy All
Author Type: Indian Author
Country: Anjanvihire, MAHARASHTRA, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2410082.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2410082
About Publisher
Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)
UGC CARE JOURNAL PUBLICATION | ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJNRD (IJ Publication) Janvi Wave | IJNRD.ORG | IJNRD.COM | IJPUB.ORG
Copyright & License
© 2025 — Authors hold the copyright of this article. This work is licensed under a Creative Commons Attribution 4.0 International License. and The Open Definition.
You are free to share, adapt, and redistribute the material, provided proper credit is given to the original author. 🛡️ Disclaimer: The content, data, and findings in this article are based on the authors’ research and have been peer-reviewed for academic purposes only. Readers are advised to verify all information before practical or commercial use. The journal and its editorial board are not liable for any errors, losses, or consequences arising from its use.
Publication Timeline
Article Preview: View Full Paper
Call For Paper
IJNRD is a Scholarly Open Access, Peer-Reviewed, Refereed, and UGC CARE Journal Publication with a High Impact Factor of 8.76 (calculated by Google Scholar & Semantic Scholar | AI-Powered Research Tool). It is a Multidisciplinary, Monthly, Low-Cost, and Transparent Peer Review Journal Publication that adheres to the UGC CARE 2025 Peer-Reviewed Journal Policy and aligns with Scopus Journal Publication standards to ensure the highest level of research quality and credibility.
IJNRD offers comprehensive Journal Publication Services including indexing in all major databases and metadata repositories, Digital Object Identifier (Crossref DOI) assignment for each published article with additional fees, citation generation tools, and full Open Access visibility to enhance global research reach and citation impact.
The INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (IJNRD) aims to advance applied, theoretical, and experimental research across diverse academic and professional fields. The journal promotes global knowledge exchange among researchers, developers, academicians, engineers, and practitioners, serving as a trusted platform for innovative, peer-reviewed journal publication and scientific collaboration.
Indexing Coverage: Google Scholar, SSRN, ResearcherID-Publons, Semantic Scholar (AI-Powered Research Tool), Microsoft Academic, Academia.edu, arXiv.org, ResearchGate, CiteSeerX, ResearcherID (Thomson Reuters), Mendeley, DocStoc, ISSUU, Scribd, and many other recognized academic repositories.
How to submit the paper?
By Our website
Click Here to Submit Paper Online
You can now publish your research in IJNRD. IJNRD is a Transparent Peer-Reviewed Open Access Journal Publication (Refereed Journal), aligning with New UGC and UGC CARE recommendations.
For more details, refer to the official notice: UGC Public Notice
Submit Paper Online
Important Dates for Current issue
Paper Submission Open For: November 2025
Current Issue: Volume 10 | Issue 11 | November 2025
Impact Factor: 8.76
Last Date for Paper Submission: Till 30-Nov-2025
Notification of Review Result: Within 1-2 Days after Submitting paper.
Publication of Paper: Within 01-02 Days after Submititng documents.
Frequency: Monthly (12 issue Annually).
Journal Type: IJNRD is an International Peer-reviewed, Refereed, and Open Access Journal with Transparent Peer Review as per the new UGC CARE 2025 guidelines, offering low-cost multidisciplinary publication with Crossref DOI and global indexing.
Subject Category: Research Area
Call for Paper: More Details
Approval, Licenses and Indexing: More Details
