GENOMIC STUDY OF ECHINOCOCCUS
CANADNESIS ISOLATED FROM CAMELS
RAISED IN SUDAN
Cystic echinococcosis CE) is considered a re-emerging disease in various regions, like Middle East, central Asia, and northern and eastern Africa. In sub-Saharan Africa, CE is highly endemic. In Sudan, high prevalence estimates of CE in both livestock and definitive host were reported. Strain variation and genetic diversity is still in need to be elucidated in this area. The aim of this study is to genotype and to study the phylogenetic relations and taxonomic status of the Echinococcus species. Total of 418 hydatid cysts collected from abattoirs survey from camel and cattle isolates,12 isolates from camels in Tamboul area and 18 isolates from cattle’s collected from (10,6, and 2 from, Nyala, Addein, and Kass, respectively), isolates( of Echinococcus (Camelus dromedaries) were collected from ( Tamboul, Nyala and Addein areas, Sudan .Molecular identification of Echinococcus species was determined by specific G5/6/7 genotype PCR and G6/7 genotype specific PCR using 1073-1078 bp and 254 bp of mtDNA respectively. Nad1, 12srRNA gene 10 isolates (2,8 respectively) for DNA sequencing was conducted. 5/6/7 genotype PCR and G6/G7 genotypes PCR reveal characteristic bands for both specific PCR. Sequences alignments reported a novel mutations of Nad1, cattle isolates of 12srRNA gene shown 98% Identity within data base blasting between Gene bank E.ortleppi isolates, that have both and suggest that the Nad1 gene are continuing to evolve in the face of current taxonomy profile. Very few bp exchanges differentiate G6 and G7, and ‘intermediate’ haplotypes have been observed, which merge them into a single genotype G6/7. A further molecular survey is needed to explore the situation of Echinococcus genotypes from human patients in Sudan.
"GENOMIC STUDY OF ECHINOCOCCUS
CANADNESIS ISOLATED FROM CAMELS
RAISED IN SUDAN", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.9, Issue 10, page no.a920-a928, October-2024, Available :https://ijnrd.org/papers/IJNRD2410100.pdf
Volume 9
Issue 10,
October-2024
Pages : a920-a928
Paper Reg. ID: IJNRD_301155
Published Paper Id: IJNRD2410100
Downloads: 00036
Research Area: Biological Science
Country: , Wad Madani, Gezira State, Sudan
DOI: http://doi.one/10.1729/Journal.41904
ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJNRD (IJ Publication) Janvi Wave
