GENOMIC STUDY OF ECHINOCOCCUS CANADNESIS ISOLATED FROM CAMELS RAISED IN SUDAN

Registration ID: IJNRD_301155

Published ID: IJNRD2410100

: http://doi.one/10.1729/Journal.41904

Ahmed Madani Elhag

Cystic echinococcosis CE) is considered a re-emerging disease in various regions, like Middle East, central Asia, and northern and eastern Africa. In sub-Saharan Africa, CE is highly endemic. In Sudan, high prevalence estimates of CE in both livestock and definitive host were reported. Strain variation and genetic diversity is still in need to be elucidated in this area. The aim of this study is to genotype and to study the phylogenetic relations and taxonomic status of the Echinococcus species. Total of 418 hydatid cysts collected from abattoirs survey from camel and cattle isolates,12 isolates from camels in Tamboul area and 18 isolates from cattle’s collected from (10,6, and 2 from, Nyala, Addein, and Kass, respectively), isolates( of Echinococcus (Camelus dromedaries) were collected from ( Tamboul, Nyala and Addein areas, Sudan .Molecular identification of Echinococcus species was determined by specific G5/6/7 genotype PCR and G6/7 genotype specific PCR using 1073-1078 bp and 254 bp of mtDNA respectively. Nad1, 12srRNA gene 10 isolates (2,8 respectively) for DNA sequencing was conducted. 5/6/7 genotype PCR and G6/G7 genotypes PCR reveal characteristic bands for both specific PCR. Sequences alignments reported a novel mutations of Nad1, cattle isolates of 12srRNA gene shown 98% Identity within data base blasting between Gene bank E.ortleppi isolates, that have both and suggest that the Nad1 gene are continuing to evolve in the face of current taxonomy profile. Very few bp exchanges differentiate G6 and G7, and ‘intermediate’ haplotypes have been observed, which merge them into a single genotype G6/7. A further molecular survey is needed to explore the situation of Echinococcus genotypes from human patients in Sudan.

Published Paper   E-Certificate

Ahmed Madani Elhag (October-2024). GENOMIC STUDY OF ECHINOCOCCUS CANADNESIS ISOLATED FROM CAMELS RAISED IN SUDAN. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 9(10), a920-a928. http://doi.one/10.1729/Journal.41904

Volume 9 Issue 10, October-2024

Pages : a920-a928

Paper Reg. ID: IJNRD_301155

Published Paper Id: IJNRD2410100

Downloads: 000122254

Research Area: Biological Science

Author Type: Foreign Author

Country: , Wad Madani, Gezira State, Sudan

Published Paper PDF: https://ijnrd.org/papers/IJNRD2410100.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2410100

Crossref DOI: http://doi.one/10.1729/Journal.41904

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

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