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A Short Review : HPLC

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Registration ID: IJNRD_303283

Published ID: IJNRD2501116

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Keywords

Introduction , Principle , Types , Overview, Basic HPLC Components , Apparatus , Separation , How to read chromatogram and Applications

Abstract

One essential analytical method that is frequently used in both qualitative and quantitative analysis is high performance liquid chromatography (HPLC), which is especially useful for estimating biological and pharmaceutical substances. One of the most sophisticated chromatographic techniques, high-performance liquid chromatography (HPLC) is acknowledged for its adaptability, safety, dependability, and speed. It is essential to the assurance and quality control of medicinal constituents. The goal of this article is to provide a thorough overview of all the aspects of HPLC, including its foundational ideas, varieties, equipment, and wide range of applications in numerous industries. Comprehending these facets allows one to recognize the importance of HPLC in contemporary analytical science and its roles in guaranteeing the security and effectiveness of medications. These days, a wide range of industries, including pharmaceuticals, biotechnology, environmental analysis, polymer research, and the food industry, heavily rely on HPLC for separations and purifications. Its accuracy and effectiveness in dissecting intricate combinations into their component parts account for its wide range of applications. A tiny volume of liquid sample is injected into the mobile phase—a continuously running stream of liquid—during the procedure. The sample is transported by this mobile phase via a column that is full of the stationary phase's densely packed particles. The components of the sample separate according to different physical or chemical properties as a result of their varied interactions with the stationary phase as it passes through the column. Because HPLC can precisely separate and purify molecules, it is an invaluable instrument for quality control and analysis of a wide range of substances in both industry and research.

How To Cite (APA)

Pansare Akanksha Dattatraya & Nibe Vaishnavi Sopan (January-2025). A Short Review : HPLC. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 10(1), b159-b167. https://ijnrd.org/papers/IJNRD2501116.pdf

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Paper Reg. ID: IJNRD_303283

Published Paper Id: IJNRD2501116

Research Area: Pharmacy All

Author Type: Indian Author

Country: Gogalgaon, MAHARASHTRA, India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2501116.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2501116

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