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INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016 Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI) |
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| Paper Title: | Effect of Caryophyllene rich betel leaf extract on Triple Negative Breast Cancer cells: an in-silico and in-vitro approach |
| Authors Name: | Disha Mohanty , Kamaljit Nag , Luna Samanta |
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| Author Reg. ID: |
IJNRD_200362
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| Published Paper Id: | IJNRDTH00059 |
| Published In: | Volume 8 Issue 6, June-2023 |
| DOI: | http://doi.one/10.1729/Journal.35004 |
| Abstract: | Triple-Negative Breast Cancer (TNBC) is a more advanced subtype of breast cancer that does not express the estrogen receptor, progesterone receptor and HER2/neu receptor. It is a kind of breast cancer which lacks all of those receptors that are commonly found in breast cancer. Because TNBC lacks the three receptors that are commonly targeted in breast cancer treatment, it can be more difficult to treat than other subtypes of breast cancer. This underscores the importance of investigating other factors that contribute to TNBC progression and developing novel therapies to target these factors. On the other hand, there is a growing body of research aimed at identifying new targets and therapies for TNBC. This ongoing research has been focused on the anticancer effects of Caryophyllene, which has been derived from the Piper betle leaf extracts. Caryophyllene, better known as β-caryophyllene, is a cannabis derived compound, which directly binds to cannabinoid receptors. These receptors have broadly been categorized into two receptors - Cannabinoid Receptor-1 (CB1R) and Cannabinoid Receptor-2 (CB2R). This study aims to identify the protein-ligand interaction between the CB2R and Caryophyllene, through an in-silico approach. It also aims to find the stimulating parameter between the protein and the ligand through an in-vitro approach on Piper betle leaves, which can detect the anticancer effects of Caryophyllene and its potent applications in TNBC treatment. For the in-silico work, a range of bioinformatics tools was utilized, such as PyMOL, AutoDockTools-1.5.7, and AutoDock Vina. Additionally, a few websites were utilized for compound screening, including PDB Database, Pubchem and UniprotKB. For the in-vitro research, two methods, which consisted of MTT Assay and Live Dead Assay, were notably used for analyzing Piper betle leaf extract. It was discovered that upon docking with the CB2R, the β-caryophyllene ligand does not form any polar hydrogen bonds with amino acid residues. It only engages in π-π stacking interactions with hydrophobic amino acid residues, particularly with phenylalanine. The MTT & Live Dead Assay was conducted using the MDA-MB-231 cell line, and the IC50 value was observed to be 0.3%. The compound β-caryophyllene drastically decreased the number of viable cells in the MDA-MB-231 cell line. These novel techniques identify the efficacy of Caryophyllene towards the prognosis and treatment of TNBC. |
| Keywords: | β-Caryophyllene, CB2R, Triple Negative Breast Cancer, MTT, Live Dead Assay |
| Cite Article: | "Effect of Caryophyllene rich betel leaf extract on Triple Negative Breast Cancer cells: an in-silico and in-vitro approach", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.8, Issue 6, page no.738-794, June-2023, Available :http://www.ijnrd.org/papers/IJNRDTH00059.pdf |
| Downloads: | 000118769 |
| ISSN: |
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID:IJNRDTH00059 Registration ID: 200362 Published In: Volume 8 Issue 6, June-2023 DOI (Digital Object Identifier): http://doi.one/10.1729/Journal.35004 Page No: 738-794 Country: Cuttack, Odisha, India Research Area: Biological Science Publisher : IJ Publication Published Paper URL : https://www.ijnrd.org/viewpaperforall?paper=IJNRDTH00059 Published Paper PDF: https://www.ijnrd.org/papers/IJNRDTH00059 |
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ISSN: 2456-4184
Impact Factor: 8.76 and ISSN APPROVED
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