“DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METHYLCOBALAMIN, ALPHA LIPOIC ACID, PYRIDOXINE AND FOLIC ACID BY RP-HPLC IN BULK AND MARKETED DOSAGE FORM”

“DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METHYLCOBALAMIN, ALPHA LIPOIC ACID, PYRIDOXINE AND FOLIC ACID BY RP-HPLC IN BULK AND MARKETED DOSAGE FORM”

Registration ID: IJNRD_202799

Published ID: IJNRDTH00070

DOI: Click Here to Get

Dr Amol Yadavrao Ghodke , Vishal Giri , Dr B N Poul , Dr S S Patil , Dr C V Panchal

RP-HPLC, Torsemide, Zorbax C18, specificity, linearity, precision, accuracy.

A simple, specific, precise and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the quantitative estimation of Methylcobalamin, alpha lipoic acid, pyridoxine and folic acid by RP-HPLC in bulk and marketed dosage form”. The proposed RP-HPLC method was carried out on 250 mm x 4.6 mm, 5um column with mobile 0.1% o-phosphoric acid: Methanol (50:50). The retention time of methylcobalamin, alpha lipoic acid, pyridoxine and folic acid was found at 2.33 ±0.2min, 3.39 ±0.2min, 5.27 ±0.2min, 6.91 ±0.2min respectively. The method was validated for specificity, precision, accuracy, linearity and robustness. The linearity range was 10-30 ug/mL and correlation coefficient (r2) was found within the range. The mean % recovery for Torsemide was found to be 99.80.

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"“DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METHYLCOBALAMIN, ALPHA LIPOIC ACID, PYRIDOXINE AND FOLIC ACID BY RP-HPLC IN BULK AND MARKETED DOSAGE FORM”", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.8, Issue 8, page no.526-610, August-2023, Available :https://ijnrd.org/papers/IJNRDTH00070.pdf

Volume 8 Issue 8, August-2023

Pages : 526-610

Paper Reg. ID: IJNRD_202799

Published Paper Id: IJNRDTH00070

Downloads: 000121227

Research Area: Pharmacy

Country: Andur, Maharashtra, India

Published Paper PDF: https://ijnrd.org/papers/IJNRDTH00070.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRDTH00070

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

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This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition

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