“DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METHYLCOBALAMIN, ALPHA LIPOIC ACID, PYRIDOXINE AND FOLIC ACID BY RP-HPLC IN BULK AND MARKETED DOSAGE FORM”

Registration ID: IJNRD_202799

Published ID: IJNRDTH00070

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Dr Amol Yadavrao Ghodke, Vishal Giri, Dr B N Poul, Dr S S Patil , Dr C V Panchal

RP-HPLC, Torsemide, Zorbax C18, specificity, linearity, precision, accuracy.

A simple, specific, precise and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the quantitative estimation of Methylcobalamin, alpha lipoic acid, pyridoxine and folic acid by RP-HPLC in bulk and marketed dosage form”. The proposed RP-HPLC method was carried out on 250 mm x 4.6 mm, 5um column with mobile 0.1% o-phosphoric acid: Methanol (50:50). The retention time of methylcobalamin, alpha lipoic acid, pyridoxine and folic acid was found at 2.33 ±0.2min, 3.39 ±0.2min, 5.27 ±0.2min, 6.91 ±0.2min respectively. The method was validated for specificity, precision, accuracy, linearity and robustness. The linearity range was 10-30 ug/mL and correlation coefficient (r2) was found within the range. The mean % recovery for Torsemide was found to be 99.80.

Published Paper   E-Certificate

Dr Amol Yadavrao Ghodke, Vishal Giri, Dr B N Poul, Dr S S Patil , & Dr C V Panchal (August-2023). “DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METHYLCOBALAMIN, ALPHA LIPOIC ACID, PYRIDOXINE AND FOLIC ACID BY RP-HPLC IN BULK AND MARKETED DOSAGE FORM”. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 8(8), 526-610. https://ijnrd.org/papers/IJNRDTH00070.pdf

Volume 8 Issue 8, August-2023

Pages : 526-610

Paper Reg. ID: IJNRD_202799

Published Paper Id: IJNRDTH00070

Downloads: 000122075

Research Area: Pharmacy

Author Type: Indian Author

Country: Andur, Maharashtra, India

Published Paper PDF: https://ijnrd.org/papers/IJNRDTH00070.pdf

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