In vitro intensification of anthraquinones in callus cultures of Rubia cordifolia by precursor feeding
Dr. Devi Priya M.
, Dr. EA Siril
Alizarin, purpurin, anthraquinone, phenylalanine, ketoglutarate
Background: Plants are enriched with various secondary metabolites. It’s over exploitation may undermine the existence of plants in this world. So plant tissue culture technology is now emerging as a new frontier in science, as it helps to improve the production rate of metabolites in in vitro condition. Callus cultures were developed on different auxin supplemented media and the callus were maintained at the optimum concentration. Precursor feeding experiment were conducted to find the increment of AQ production under the influence of precursors used viz., ketoglutarate (KG) and phenylalanine (PA). Friable calli were produced from nodal segments cultured on MS medium containing 5 µM NAA and estimated 17.76 mg g-1 dw alizarin and 3.8 mg g-1 dw purpurin. In callus cultures addition 100 µM phenylalanine as precursor has resulted 25 fold higher alizarin production over roots sampled from naturally growing plant. This work is focused to improve the AQ production through callus culture of R. cordifolia so that we could maintain the continuous supply of AQ without disturbing the plant intact.
"In vitro intensification of anthraquinones in callus cultures of Rubia cordifolia by precursor feeding", IJNRD - INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT (www.IJNRD.org), ISSN:2456-4184, Vol.7, Issue 6, page no.369-375, June-2022, Available :https://ijnrd.org/papers/IJNRD2206041.pdf
Volume 7
Issue 6,
June-2022
Pages : 369-375
Paper Reg. ID: IJNRD_181740
Published Paper Id: IJNRD2206041
Downloads: 000118802
Research Area: Science & Technology
Country: Pathanamthitta, Kerala, India
ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJNRD (IJ Publication) Janvi Wave
