INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016
Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI)
UV and RP-HPLC method was developed for estimation of Lasmiditan in bulk and tablets dosage form by isocratically using Methanol: Acetonitrile in the ratio of 50:50 v/v as mobile phase, Thermo C-18 column (4.6 x 250mm, 5μparticle size) column as stationary phase and chromatogram was recorded at 228 nm. Then developed method was validated by using various parameters. The linearity of analytical method to elicit test results that are proportional to the concentration of analyte in sample within a given range.
Specificity of the method was determined and the peaks of diluent, mobile phase and excipient of tablets did not interfere with standard peaks Lasmiditan. The validity and reliability of proposed methods were assessed by recovery studies. The recovery of added standards (80%, 100% and 120%) was found at three replicate and three concentrations level. The value of % means just close to 100, SD and % RSD are less than 2 indicate the accuracy of method. The assay value of drugs was close to 100, SD and % RSD are less then 2 indicate the no interference of excipient in the estimation of drug
Keywords:
UV, RP-HPLC, Lasmiditan, Linearity, Accuracy
Cite Article:
"Novel high performance liquid chromatographic method development and validation for the estimation of lasmiditan in marketed formulation", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.7, Issue 9, page no.1509-1519, September-2022, Available :http://www.ijnrd.org/papers/IJNRD2209178.pdf
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ISSN:
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
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