To Study The Effect Of Temperature On Enzyme Activity.

Registration ID: IJNRD_197285

Published ID: IJNRD2305800

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Komal S. Shelar , Snehal C. Batule , Kiran B. Dhamak

β-amylase, Denaturation, Enzyme activity, Optimum Temperature.

The current study was carried out to determine the effect of temperature on enzyme activity. The activity of the enzyme was measured after incubating the crude enzyme preparation under assay conditions at temperatures ranging from 10 to 60 °C, as well as at room temperature. Foods like potatoes, soybean seeds, ginger, apples as well as saliva, are sources of the enzyme beta-amylase, which is employed in industry. These sources were used to isolate, purify, and characterised beta amylase. DNS reagent, 5% Starch, 0.5% NaCl, 0.5M NaOH, and pH 4.6 Acetate buffer were used to complete this process. Using a photocolorimeter (ESICO) and UV-Visible Spectrophotometer (SHIMADZU), the effect of temperature (absorbance) was determined. To assess the impact of temperature, we used several extracts of beta amylase in the current investigation. The majority of enzyme extracts demonstrated that, up until a maximum, the speed of an enzyme reaction increases with an increase in temperature before decreasing. Temperature coefficient, or Q10, is defined as the increase in enzyme velocity when the temperature is raised by 10°C and is typically observed as a bell-shaped curve. For a Q10 is 2 for the vast majority of enzymes between 0°C and 40°C.Most enzymes work best at a temperature of 35 to 40°C ,However there are a few enzymes. The natural (tertiary) structure of the enzymes is typically destabilised when they are exposed to temperatures above 50 °C due to denaturation. At temperatures above 70°C, the majority of enzymes become inactive. We draw the conclusion that the majority of the enzyme exhibits maximum activity at the optimum temperature and then becomes denaturized as temperature is increased based on a literature review and experimental data. Therefore the activity of each of our enzyme extracts was in accordance with the mentioned catalyst for enzyme function. Temperature increases do have an impact on enzymes. The temperature at which an enzyme starts to function and the temperature at which a protein starts to break down are therefore the two factors that dictate the range of an enzyme activity.

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Komal S. Shelar, Snehal C. Batule, & Kiran B. Dhamak (May-2023). To Study The Effect Of Temperature On Enzyme Activity.. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 8(5), h793-h801. https://ijnrd.org/papers/IJNRD2305800.pdf

Volume 8 Issue 5, May-2023

Pages : h793-h801

Paper Reg. ID: IJNRD_197285

Published Paper Id: IJNRD2305800

Downloads: 000122007

Research Area: Pharmacy

Country: Nashik., Maharashtra , India

Published Paper PDF: https://ijnrd.org/papers/IJNRD2305800.pdf

Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2305800

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar | ESTD YEAR: 2016

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