Isolation & Characterization Of Bacteria, Fungi And Parasites From Infected Migratory Birds (Kokkare Belluru Pelicans)

Registration ID: IJNRD_191939

Published ID: IJNRD2304453

: http://doi.one/10.1729/Journal.33895

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Amruthavarshini S N

Pelican, Pelecanus belonging to the Pelecanidae family are defenseless to different infection by an assortment of pathogens. They succumb to illness due its low immunity and unhealthy environment. Avian infections contribute to an important set of problems within the life cycle balancing system and live stock production. Various pathogens are known and have been studied. More studies have been done on infection that commonly caused in bovine such as Botulism, Psittacosis and few others. Fungal diseases have been studied such as Aspergillosis, Candidiasis, Cryptococcosis and few others. Parasitic diseases have also been studied such as Helminth and arthropod. Pelicans are infected by number of pathogens and they succumb to diseases. For this study, we considered the most common infections Botulinum, Aspergillosis and Helminth, Nematode (Ringworm) & Arthropod parasitic infections. By plating technique infected sample of Pelican were screened for bacteria and fungi, using Agar-Agar, Potato Paste and Sheep blood and incubated at 25’C. Once the visible colonies were formed on the plate, they were characterized by colony morphology. To further identify the fungi, mycelial studies were done. The fungi culture slide were prepared by tease mount technique using lacto phenol cotton blue stain [LPCB]. The slides were then analyzed to characterize the fungi. The identification was done as per the guidelines recommended by Larone (1976) and Al-Doory (1980). And to identify bacteria, the bacterial culture slide were prepared by Gram’s staining using Crystal violet, Gram’s iodine, 90% alcohol and Safrannin suggested by Danish bacteriologist Hans Christian Gram, who developed the technique (1882). To further identify the microbes present in water, samples were collected and plating technique was done. Molluscs as being rare food for Pelicans were also collected and examined by bacterial staining technique. Identification of the parasitic infection in Bird, were analyzed by the food chain of birds, (Pelicans feed on molluscs, arthropods which may cause parasitic infections). They sometimes even feed on maggots. Investigation on the food cycle parasitic infection detected. The organism found to be Fusarium mycotoxin, i.e., Deoxynivenol mycotoxin. Further studies have to be carried out in order to study the mechanism and role involved in pathogenesis.

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Amruthavarshini S N (April-2023). Isolation & Characterization Of Bacteria, Fungi And Parasites From Infected Migratory Birds (Kokkare Belluru Pelicans). INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 8(4), e390-e405. http://doi.one/10.1729/Journal.33895

Volume 8 Issue 4, April-2023

Pages : e390-e405

Paper Reg. ID: IJNRD_191939

Published Paper Id: IJNRD2304453

Research Area: Life Sciences 

Author Type: Indian Author

Country: CHICKBALLAPUR, Karnataka, India

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Crossref DOI: http://doi.one/10.1729/Journal.33895

Journal Name: INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT(IJNRD)

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