Paper Title
COMPARATIVE EVALUATION OF ANTIMICROBIAL ACTIVITY OF 5% ALLIUM SATIVUM, 5% SODIUM HYPOCHLORITE AND MTA AS PULPOTOMY MEDICAMENTS IN PRIMARY TEETH: AN IN VITRO STUDY.
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Registration ID: IJNRD_208232
Published ID: IJNRD2311102
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Keywords
Pulpotomy, Allium Sativum, MTA
Abstract
Aim: The Aim of the study was to compare and evaluate the antimicrobial activity of Allium sativum, mineral trioxide aggregate (MTA) and Sodium hypochlorite against E. faecalis and S. aureus as a pulpotomy medicament in primary teeth. Objective: To compare and evaluate the antibacterial activity of herbal plant extract allium sativum oil (garlic oil), mineral trioxide aggregate (MTA) and sodium hypochlorite (Naocl) against E. faecalis and S. aureus as a pulpotomy medicament in primary teeth. Material and method: Total 120 samples were taken and divided into three groups. Group – 1 was taken as a control group with 24 samples in which 5% Sodium hypochlorite was used. Group - 2 was taken as experimental group with 72 samples which were further subdivided into 3 groups of 24 samples each in which 5%, 50%, 80% Allium sativum oil was used and group – 3 was taken as experimental group with 24 samples in which Mineral trioxide aggravates (MTA Angelus) was used, as pulpotomy medicament in primary teeth using an agar diffusion method. Antimicrobial activities of the 5% Sodium hypochlorite, 5%, 50%, 80% Allium sativum oil and Mineral trioxide aggravates was evaluated against staphylococcus aureus and Enterococcus faecalis using agar diffusion method. All the microbial strains were grown at 37°C at 24 hours in Mueller-Hinton broth. The plates were kept at room temperature for 2 hours for the pre diffusion of materials and then Incubated at 37°C for 24 hours and 48 hours respectively. After that, inhibition zones were measured after 24 and 48 hours by using a digital calliper. Measurements were taken at the greatest distance between two points at the outer limit of the inhibition halo formed around the well. Result: Statistical analysis was done by one way analysis of variance to check the difference between the group and post hoc analysis was done by turkey’s test. The result showed that the comparison of Staphylococcus aureus after 24 hours between the five groups was statistically significant in which p value was <0.001 and after 48 hours comparison was not Significant with a statistics p value of 0.207 and in Enterococcus Faecalis group comparison between five groups after 24 hours showed statistically Significant result with a test value of <0.001 and after 48 hours this difference was not statistically significant with a test value was 0.135. Conclusion: In the present study we observed that 50% and 80% allium sativum, MTA and 5% sodium hypochlorite have antimicrobial activity against Staphylococcus aureus and Enterococcus faecalis. However, the concentration of 5% allium sativum is not so effective on the Enterococcus faecalis. So garlic extract in the concentration of 50% and 80% could be an alternative to commercially available pulpotomy agent due to its cost effectiveness and minimum side effects.
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Dr.Jalpa Solanki, Dr. Vikram Jhamb, Dr. shital kiran D.P., Dr. Shalin Shah, & Dr. Malay Trivedi (November-2023). COMPARATIVE EVALUATION OF ANTIMICROBIAL ACTIVITY OF 5% ALLIUM SATIVUM, 5% SODIUM HYPOCHLORITE AND MTA AS PULPOTOMY MEDICAMENTS IN PRIMARY TEETH: AN IN VITRO STUDY.. INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT, 8(11), b8-b18. https://ijnrd.org/papers/IJNRD2311102.pdf
Issue
Volume 8 Issue 11, November-2023
Pages : b8-b18
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Paper Reg. ID: IJNRD_208232
Published Paper Id: IJNRD2311102
Research Area: Medical Science
Author Type: Indian Author
Country: Bhavnagar, Gujarat, India
Published Paper PDF: https://ijnrd.org/papers/IJNRD2311102.pdf
Published Paper URL: https://ijnrd.org/viewpaperforall?paper=IJNRD2311102
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