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IJNRD
INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT
International Peer Reviewed & Refereed Journals, Open Access Journal
ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016
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Impact Factor : 8.76

Issue per Year : 12

Volume Published : 8

Issue Published : 80

Article Submitted : 7144

Article Published : 2923

Total Authors : 7078

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Paper Title: Effect of Annona Muricata on CD8 and Granzyme-b Expression in Severe Plasmodium berghei Anka Infected Swiss Mice
Authors Name: Abdulkarim Abdullah Mohammed Amammish , Kis Djamiatun , Muflihatul Muniroh , Dik Puspasari
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IJNRD_180329
Published Paper Id: IJNRD2008038
Published In: Volume 5 Issue 8, August-2020
DOI:
Abstract: Several studies have reported the role of T cells in Plasmodium berghei ANKA (PbA) that related with the blood and liver patology. Granzyme B (GzmB) is a serine protease released by CD8+ T cells and natural killer cells during cellular immune response, The purpose of these study to determine the effect of Annona muricata leaf-ethanolic exract (AME) on GzmB and CD8-expression-score in spleens of PbA-infected-Swiss-mice. This experimental study used randomized post test only control group design. Thirty six female Swiss mice were divided into 6 groups. C1-group was control-healthy mice, G1 and G2-groups were healthy-mice treated with AME dosage 100 and 150 mg/kg BW/day for 14-day-period. C2-group was control-PbA-infected-group. G3 and G4-groups received AME dosage 100 and 150 mg/kg BW, respectively for 7-day-pre and post-PbA-infection. GzmB and CD8 in the spleen stained by immunohistochemistry-method, then two-pathology-anatomy-experts determined Allred-score by using light microscope. Statistical analyzes used were One-way-ANOVA-test followed by post-hoc-test, and. Pearson-correlation-test. G4 and C1-groups had no significant different GzmB-expression-score (5.60 ± 0.46 and 4.10 ± 1.27; p = 0.134), while those of G3 and C2-groups (6.03 ± 0.48 and 6.70 ± 0.99) were significantly higher than C1-group (p = 0.015 and p = 0.012). AME (150mg/kg BW/day)-treatment associated with GzmB-expression-normal score during severe-PbA-infection. G3, G4 and C2-groups (5.03 ± 0.79; 6.20 ± 1.46 and 6.60 ± 2.77) had no significant different CD8-expression-score compare to C1-group (3.80 ± 2.60; p = 0.829; p = 0.284 and p = 0.150). The correlation score of GzmB and CD8 expression was not found in healthy Swiss mice infected with PbA (r = -0.248; p = 0.158). AME in dose of 150 mg/kg BW/day-treatment normalize GzmB-expression-score of severe-PbA-infected-Swiss-mice, however AME at any dose studied have no influence on CD8- expression-score of those mice
Keywords: Annona muricata, P. berghei Anka, granzyme B, CD8
Cite Article: "Effect of Annona Muricata on CD8 and Granzyme-b Expression in Severe Plasmodium berghei Anka Infected Swiss Mice", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.5, Issue 8, page no.338-346, August-2020, Available :http://www.ijnrd.org/papers/IJNRD2008038.pdf
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ISSN: 2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publication Details: Published Paper ID:IJNRD2008038
Registration ID: 180329
Published In: Volume 5 Issue 8, August-2020
DOI (Digital Object Identifier):
Page No: 338-346
Country: Semarang, Indonesia, Indonesia
Research Area: Medical Science
Publisher : IJ Publication
Published Paper URL : https://www.ijnrd.org/viewpaperforall?paper=IJNRD2008038
Published Paper PDF: https://www.ijnrd.org/papers/IJNRD2008038
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ISSN: 2456-4184
Impact Factor: 8.76 and ISSN APPROVED
Journal Starting Year (ESTD) : 2016

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