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INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016 Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI) |
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| Paper Title: | Production and Optimization of Polyhydroxy butyrate-(PHB) from Bacterial isolates. |
| Authors Name: | Rajvi Patel , Nirali Patel , Hetal Panchal |
| Download E-Certificate: | Download |
| Author Reg. ID: |
IJNRD_182186
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| Published Paper Id: | IJNRD2207132 |
| Published In: | Volume 7 Issue 7, July-2022 |
| DOI: | http://doi.one/10.1729/Journal.31118 |
| Abstract: | PHBs are storage material synthesize by different microorganisms.The present study was focused on Production of PHB by bacterial isolates isolated from local soil samples.All the bacterial isolates were screened for their PHB producing ability and two isolates showing highest PHB production were selected for further studies.Optimization of different variables like pH, temperature, incubation period, carbon and nitrogen sources were performed.The maximum PHB accumulation was found when the medium was supplemented with glucose and sucrose as a carbon source. Yeast extract and peptone were found optimum nitrogen source.The optimum pH and temperature were 6.5-7.0 and 35°C respectively. The polymer can be further purified by dissolving in chloroform and precipitating with cold ethanol. PHB extraction carried out using sodium hypochlorite method exhibited a good PHB yield amounting to 73% and 80% for S1b and S3b isolates respectively. The extracted compounds on FTIR analysis gave characteristics functional units as C-H, CH2 ,-OH,C=O groups. UV-Vis spectrophotometric analysis provide confirmed similarity of extracted PHB and its suitability for bioplastic production. |
| Keywords: | Bioplastic, Polyhydroxybutyrate-(PHB), Optimization, FTIR spectroscopy |
| Cite Article: | "Production and Optimization of Polyhydroxy butyrate-(PHB) from Bacterial isolates.", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.7, Issue 7, page no.1148-1157, July-2022, Available :http://www.ijnrd.org/papers/IJNRD2207132.pdf |
| Downloads: | 000118757 |
| ISSN: |
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID:IJNRD2207132 Registration ID: 182186 Published In: Volume 7 Issue 7, July-2022 DOI (Digital Object Identifier): http://doi.one/10.1729/Journal.31118 Page No: 1148-1157 Country: Valsad, Gujrat, India Research Area: Biological Science Publisher : IJ Publication Published Paper URL : https://www.ijnrd.org/viewpaperforall?paper=IJNRD2207132 Published Paper PDF: https://www.ijnrd.org/papers/IJNRD2207132 |
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ISSN: 2456-4184
Impact Factor: 8.76 and ISSN APPROVED
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