INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016
Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI)
Aim: To isolate endophytic microorganisms from the leaves of Ocimum sanctum, Plectranthus amboinicus, and from the flower of Hibiscus rosa-sinensis..To assess and comparatively study the antibacterial effect of secondary metabolites of endophytic bacteria and fungi.
Methods: Surface sterilization of collected samples, pre-treatment of sterilized samples, isolation, and preliminary characterization of these endophytes was carried out via microscopic observations and biochemical characterization. These endophytes were screened for their antibacterial activity.
Results: A total of 3 fungi and 31 bacteria were isolated from these medicinal plant samples. Out of which only three fungal and four bacterial endophytes were chosen based on the agar plate activity, sub-cultured and screened for the production of bioactive compounds. Later the effect of their secondary metabolites was studied against multidrug-resistant bacteria such as Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Serretia sp. All the chosen endophytes except one fungi showed promising inhibitory effects against the test organisms.
Conclusion: The zones of inhibition obtained from the fungal metabolites were wider and had better inhibitory effect on all the test organisms even at a lower concentrations where as, the secondary metabolites obtained from the four bacterial endophytes displayed as varying effect on the above mentioned organisms. Hence its confirmed that the fungal metabolites are more efficient in controlling the growth of these test organisms. The increased concentrations of the bacterial metabolites enhances its antibacterial effects.
"Endophytes From Medicinal Plants as Biocontrol Agents ; A Comparative Study", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.7, Issue 10, page no.183-194, October-2022, Available :http://www.ijnrd.org/papers/IJNRD2210147.pdf
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2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
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