|
|||||||||||||||
|
INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016 Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI) |
||||||||||||||
Issue: May 2024
Volume 9 | Issue 5
Review Result and Publication of Paper within : 2-3 days
Click Here For more DetailsFor Authors
Forms / Download
Published Issue Details
Editorial Board
Other IMP Links
Facts & Figure
Impact Factor : 8.76
Issue per Year : 12
Volume Published : 9
Issue Published : 96
Article Submitted :
Article Published :
Total Authors :
Total Reviewer :
Total Countries :
Indexing Partner
Join RMS/Earn 300
Licence
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
|
Published Paper Details
|
|
| Paper Title: | UPLC method Validation for bioanalysis of Gemcitabine in rat PK study using VAMS methodology (Volumetric Absorptive Microsampling) |
| Authors Name: | Raja Rajeswari Katta , HAFIS JAMAL AYYIL , S.Kathirvel |
| Download E-Certificate: | Download |
| Author Reg. ID: |
IJNRD_194081
|
| Published Paper Id: | IJNRD2305278 |
| Published In: | Volume 8 Issue 5, May-2023 |
| DOI: | |
| Abstract: | Volumetric absorptive microsampling (VAMS) is a simple intuitive technique for collecting and quantitative analysis of dried blood samples. It enables the collection of an accurate blood volume regardless of blood hematocrit. A bioanalytical method for the determination of gemcitabine in dried blood supported on VAMS samplers has been validated and used to support a pharmacokinetic study in rat. The calculated PK parameters were comparable to those obtained from blood–water (1:1, v/v) samples. VAMS is demonstrated to be a robust method that simplifies both the blood sample collection and bioanalytical laboratory procedures and generates high quality quantitative data. Waters Acquity UPLC system using an Acquity BEH C18 column (100x2.1 mm, 1.7 μm) was used for chromatographic separation by isocratic elution using acetonitrile-water (35-65) as the mobile phase at a flow rate of 0.5 mL/min. Gemcitabine was administered to rat orally at 3 mg/kg for conducting the PK study and the blood was collected at various time intervals using VAMS sampler which consists of a hydrophilic polymeric tip, absorbs an accurate sample volume within 2–4 s by wicking, attached to a molded plastic handle. The tip is white before use and turns completely red when filled with blood, and the blood samples were processed after collection and analyzed by UPLC. The intra-day and inter-day accuracy of gemcitabine were 93.6–108.7% and 94.2–111.5% respectively, and the precision (RSD, %) was less than 15% for both intra-day and inter-day measurements. Gemcitabine has a good linear relationship in the range of 50-500 ng/mL with r2 value of 0.997. A robust and reliable UPLC method was fully optimized and developed to detect the blood concentration of gemcitabine in rats and the samples were analyzed by Empower software. |
| Keywords: | Gemcitabine, VAMS, UPLC, Validation, Bioanalytical, Pharmacokinetics |
| Cite Article: | "UPLC method Validation for bioanalysis of Gemcitabine in rat PK study using VAMS methodology (Volumetric Absorptive Microsampling) ", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.8, Issue 5, page no.c607-c630, May-2023, Available :http://www.ijnrd.org/papers/IJNRD2305278.pdf |
| Downloads: | 000118755 |
| ISSN: |
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
| Publication Details: |
Published Paper ID:IJNRD2305278 Registration ID: 194081 Published In: Volume 8 Issue 5, May-2023 DOI (Digital Object Identifier): Page No: c607-c630 Country: Kozhikode, Kerala, India Research Area: Pharmacy Publisher : IJ Publication Published Paper URL : https://www.ijnrd.org/viewpaperforall?paper=IJNRD2305278 Published Paper PDF: https://www.ijnrd.org/papers/IJNRD2305278 |
| Share Article: |
|
|
Click Here to Download This Article |
|
| Article Preview | |
|
|
|
Major Indexing from www.ijnrd.org
| Semantic Scholar | Microsaoft Academic | ORCID | Zenodo |
| Google Scholar | ResearcherID Thomson Reuters | Mendeley : reference manager | Academia.edu |
| arXiv.org : cornell university library | Research Gate | CiteSeerX | PUBLON |
| DRJI | SSRN | Scribd | DocStoc |
ISSN Details
 |
 |
|
ISSN: 2456-4184
Impact Factor: 8.76 and ISSN APPROVED
Journal Starting Year (ESTD) : 2016
DOI (A digital object identifier)
Conference
Open Access License Policy
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Important Details
Social Media
Licence
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Join RMS/Earn 300
| Copyright © 2024 - All Rights Reserved - IJNRD |
Facebook Twitter Instagram LinkedIn