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INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016 Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI) |
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| Paper Title: | Isolation and screening of bacteria from Jeevamrutha and Ghanajeevamrutha against the pathogen Sclerotium rolfsii for their biocontrol activity and their biochemical characterization |
| Authors Name: | SAHANA S.B. , SREENIVASA M.N. , C.R. PATIL , Gurudatt M. Hegde |
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IJNRD_194610
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| Published Paper Id: | IJNRD2306087 |
| Published In: | Volume 8 Issue 6, June-2023 |
| DOI: | |
| Abstract: | Abstract: An experiment was conducted to study the biocontrol activity of bacterial isolates from Jeevamrutha and Ghanajeevamrutha at the University of Agricultural Sciences, Dharwad. Jeevamrutha and Ghanajeevamrutha are cheaper and eco-friendly organic preparations made of desi cow products. These are efficient plant growth stimulants that enhance the biological efficiency of crops. Soil borne plant pathogens cause serious losses to crop plants reducing the yield to the extent of 75-80 per cent. In this context, bacteria were isolated from the Jeevamrutha and Ghanajeevamrutha (prepared at laboratory scale) and were subjected for dual culture plate assay on Potato Dextrose Agar medium against the plant pathogen Sclerotium rolfsii. Zone of inhibition was calculated by comparing to the pathogen growth on the control plate. The isolated bacteria showed different degrees of antagonism (28.75-47.78 per cent) against the pathogen, among them the isolates J1 (isolate from Jeevamrutha) and GJ1(isolated from Ghanajeevamrutha) showed significantly the highest inhibition percentage of 47.78 per cent and 36.55 per cent respectively against the pathogen Sclerotium rolfsii. Further the isolates were subjected for morphological and biochemical characterization, the bacterial isolates were found to be a mixture of both Gram positive and Gram-negative bacteria with varying shapes. Most of them were positive for citrate utilization test indicating citrate as the sole source of carbon used by the isolates, only few were positive for PAL test i.e., Phenylalanine ammonia lyase test. |
| Keywords: | Keywords: Jeevamrutha, Ghanajeevamrutha, Sclerotium rolfsii, per cent inhibition, biochemical characterization. |
| Cite Article: | "Isolation and screening of bacteria from Jeevamrutha and Ghanajeevamrutha against the pathogen Sclerotium rolfsii for their biocontrol activity and their biochemical characterization", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.8, Issue 6, page no.a754-a764, June-2023, Available :http://www.ijnrd.org/papers/IJNRD2306087.pdf |
| Downloads: | 000118753 |
| ISSN: |
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID:IJNRD2306087 Registration ID: 194610 Published In: Volume 8 Issue 6, June-2023 DOI (Digital Object Identifier): Page No: a754-a764 Country: dharwad , karnataka, India Research Area: Life Sciences Publisher : IJ Publication Published Paper URL : https://www.ijnrd.org/viewpaperforall?paper=IJNRD2306087 Published Paper PDF: https://www.ijnrd.org/papers/IJNRD2306087 |
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ISSN: 2456-4184
Impact Factor: 8.76 and ISSN APPROVED
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