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INTERNATIONAL JOURNAL OF NOVEL RESEARCH AND DEVELOPMENT International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2456-4184 | Impact factor: 8.76 | ESTD Year: 2016 Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.76 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI) |
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| Paper Title: | “DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METHYLCOBALAMIN, ALPHA LIPOIC ACID, PYRIDOXINE AND FOLIC ACID BY RP-HPLC IN BULK AND MARKETED DOSAGE FORM” |
| Authors Name: | Dr Amol Yadavrao Ghodke , Vishal Giri , Dr B N Poul , Dr S S Patil , Dr C V Panchal |
| Download E-Certificate: | Download |
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IJNRD_202799
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| Published Paper Id: | IJNRDTH00070 |
| Published In: | Volume 8 Issue 8, August-2023 |
| DOI: | |
| Abstract: | A simple, specific, precise and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the quantitative estimation of Methylcobalamin, alpha lipoic acid, pyridoxine and folic acid by RP-HPLC in bulk and marketed dosage form”. The proposed RP-HPLC method was carried out on 250 mm x 4.6 mm, 5um column with mobile 0.1% o-phosphoric acid: Methanol (50:50). The retention time of methylcobalamin, alpha lipoic acid, pyridoxine and folic acid was found at 2.33 ±0.2min, 3.39 ±0.2min, 5.27 ±0.2min, 6.91 ±0.2min respectively. The method was validated for specificity, precision, accuracy, linearity and robustness. The linearity range was 10-30 ug/mL and correlation coefficient (r2) was found within the range. The mean % recovery for Torsemide was found to be 99.80. |
| Keywords: | RP-HPLC, Torsemide, Zorbax C18, specificity, linearity, precision, accuracy. |
| Cite Article: | "“DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METHYLCOBALAMIN, ALPHA LIPOIC ACID, PYRIDOXINE AND FOLIC ACID BY RP-HPLC IN BULK AND MARKETED DOSAGE FORM”", International Journal of Novel Research and Development (www.ijnrd.org), ISSN:2456-4184, Vol.8, Issue 8, page no.526-610, August-2023, Available :http://www.ijnrd.org/papers/IJNRDTH00070.pdf |
| Downloads: | 000118764 |
| ISSN: |
2456-4184 | IMPACT FACTOR: 8.76 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.76 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID:IJNRDTH00070 Registration ID: 202799 Published In: Volume 8 Issue 8, August-2023 DOI (Digital Object Identifier): Page No: 526-610 Country: Andur, Maharashtra, India Research Area: Pharmacy Publisher : IJ Publication Published Paper URL : https://www.ijnrd.org/viewpaperforall?paper=IJNRDTH00070 Published Paper PDF: https://www.ijnrd.org/papers/IJNRDTH00070 |
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ISSN: 2456-4184
Impact Factor: 8.76 and ISSN APPROVED
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